The aim of this project was to investigate into the effect of the Loa mutation in the function of the cytoplasmic dynein complex. Protein-protein interaction assays were performed on NIH3T3, NSC34 and HEK293T cells transfected with a 2 kb fragment of the wild type or mutant dynein heavy chain gene 1 bearing the Loa mutation and seven different intermediate chain isoforms. The results indicated a 2.5 fold increase in the binding affinity of the mutant dynein heavy chain 1 727 amino acid fragment for the dynein intermediate chain gene 2 isoform A, compared to the wild type.