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Title: Effects of different assay configurations on pharmacological profiling of GPCR targets
Author: Mason, Vicki Louise
ISNI:       0000 0001 3620 8442
Awarding Body: The University of Reading
Current Institution: University of Reading
Date of Award: 2007
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The effect of assay type and assay configuration on the pharmacological profile of a range of ligands acting at the D2 dopamine receptor was investigated in this thesis. The ligands explored have previously been shown to display a spectrum of efficacy at the D2 dopamine receptor. In competition ligand binding studies versus [³H]spiperone in HEK-293CREβlacD₂ˡ cell membranes, receptor-G protein coupling was increased in the absence of Na⁺, however the pK/ values for some agonists were higher in the presence of Na⁺, which suggested that different ligands are able to induce different receptor conformations.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available