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Title: Developments in flourescence measurements of NADH
Author: Haramis, Helena
ISNI:       0000 0001 3531 1907
Awarding Body: Manchester University
Current Institution: University of Manchester
Date of Award: 2003
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The objectives of this research included the development of a novel imaging set-up for fluorescence monitoring of nicotinamide adenine dinucleotide (NADH), in order to investigate the metabolic state of tissues and hence viability in a number of pathological conditions. We validated our system using different concentrations of NADH in the presence and absence of various optical scatterers and absorbers. Fluorescence images of dilute and turbid tissue phantom samples were acquired at an excitation of 366 nm, specific for NADH. These studies were followed by investigations ex vivo and in vivo: (a) to determine if NADH could be used as an indicator of ischaemia in hydrocephalus resulting from an increase in intracranial pressure due to an accumulation of cerebrospinal fluid; (b) monitor the effects of decreased blood flow on metabolic function of xanografted solid tumours in response to an antitumour compound; and (c) if measurement of NADH can be used as an indicator of changes in metabolic activity induced by pentobarbital.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available