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Title: Controlled substrate release for optimising arenes' biotransformation to cis-dihydrodiols
Author: Boudet, Cyril Jean
ISNI:       0000 0001 3471 8932
Awarding Body: Queen's University of Belfast
Current Institution: Queen's University Belfast
Date of Award: 2008
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This project studies the effect of cation and anion stmctures of ionic liquids (ll..) phase on whole cell biocatalysts that enable the biotransformation of mono-halogenated benzene into cis-hydroxydiol products by mainly Pseudomonas plltida UV4 (P. pUlida UV4) expressing a toluene dio},.)'genase. The biotransformations in the presence ofll..s were investigated with imidazolium and pyrrolidinium based cations and bis(trifluoromethanesulfonyl)amide acting as anion. Increased conversion of substrate and enhancement ofthe biotransformation rate with P. putida UV4 was observed as the n-alkyl chain length of the cation was increased. This led us to study the compatibility of these ll.. phases to enhance biotransfonl1ation with an H. coli TDO clone and S. yalloikuyae B8/36. The synthesis of different lLs was investigated with side chains designed to improve compatibility and enhance· biotransformation with 1)'. yanoikuyae B8/36. This effect was thought to be due to direct delivery of the substrate from the IL phase to the cell without transfer through the aqueous phase. However, we have also considered that ILs possibly inhibit enzymatic reactions in the cell membrane. The inhibition of the cell respiration monitored with an oJl..)'gen electrode during exposition of cell to IL phases was investigated and compared to specific inhibitors to detemline the mechanism ofthis biotransformation enhancement. Supplied by The British Library - 'The world's knOWledge'
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available