Because of its central role in purine and thymidylate biosynthesis, folate-mediated one-carbon metabolism has been the target of many anticancer drug therapies. The objective of this study is to utilise the structural similarity of the anticancer drug, nnethotrexate (MTX) to folate, and optimise chemical-based genetic screens for the isolation of folate transporters and/or elements important for folate homeostasis in Arabidopsis thaliana.