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Title: Harnessing the potential of gaseous ozone for the disinfection of fresh produce in storage and transit
Author: Roberts, Raveenia Ellisa
ISNI:       0000 0001 3525 2951
Awarding Body: Newcastle University
Current Institution: University of Newcastle upon Tyne
Date of Award: 2005
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Freshly-harvested fruit and vegetables are prone to microbial spoilage sustained during production, transportation and storage. Traditional chemical treatments, including sanitizing agents such as chlorinated compounds, fail to adequately control many of the common microbes responsible for spoilage as wen as potentially harmful human pathogens. As a result, safe, effective alternatives to current practices are urgently required to reduce spoilage, Improve food safety and gain consumer confidence. Research and commercial applications have established that ozone, a powerful oxidant, may provide a viable alternative to traditional disinfectants. Using fruit of tomato, Lycopersicon esculentum, L., as an experimental model the work reported in this thesis was undertaken to explore the efficacy of gaseous ozone for the preservation of fresh produce in storage and transit. Findings illustrate that extremely low-levels of ozone enrichment, 0.05 ppm, were significant in reducing surface microtlora and Botrytis cinerea lesion development. Moreover, ozone-treated fruit were effectively 'vaccinated' against subsequent infection, and this effect persisted for up to 2 weeks after fruit were removed from an ozone-enriched environment. Interestingly, RT-PCR assays showed that ozone repressed the expression of several defence-related genes and detectable shifts in gene expression mirrored the duration of 'memory effects'. Ozone treatment resulted in no deleterious effects on fruit quality. Indeed, fresh weight loss was 50% of that experienced by non-ozonated fruit and there appeared to be detectable benefits of ozone treatment on taste and appearance. Key words: gaseous ozone, fresh produce, spoilage losses, storage, transit.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available