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Title: A clinical investigation of circulating endothelial progenitor cells in cardiovascular repair
Author: Thomas, Honey
ISNI:       0000 0001 3522 2816
Awarding Body: Newcastle University
Current Institution: University of Newcastle upon Tyne
Date of Award: 2008
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Background Endothelial progenitor cells (EPCs) are circulating progenitor cells involved in vascular repair and regeneration. I investigated the role of EPCs in cardiac transplant recipients, to establish whether reduced EPCs are associated with cardiac allograft vasculopathy (CAV), and to investigate whether patients receiving older donor hearts have lower EPCs than those receiving hearts from younger donors. I carried out a study to 'determine whether controlled vascular damage occurring at percutaneous coronary intervention (pCI) results in EPC release. Finally I assessed whether EPCs show diurnal variation in healthy individuals. Methods and Results I used flow cytometry quantification of absolute EPC counts with all the commonly used phenotypes from whole peripheral blood. The methods underwent extensive optimisation stages and reproducibility studies. I did not find any difference EPC numbers between cardiac transplant patients with and without CAV. There was no reduction in EPCs in patients with older donor hearts. There was no evidence of EPC mobilisation in the first 24 hours after PCI, rather there was a fall in EPCs in the first 6 hours. This is similar to the pattern ofdiurnal variation found in healthy volunteers sampled at 3 time points who showed a fall in EPCs between 8am and 3pm and a subsequent rise at lOpm. Conclusions My work has shown that EPCs are not reduced in CAV which highlights the pathophysiological differences from coronary disease. It provides evidence supporting the theory that age related decline in EPCs is not due to aging of the target tissues. also challenge the existence of a vascular injury specific mobilisation ofEPCs following PCI. Finally I have demonstrated a significant diurnal variation in EPCs which is important both in underst&lding EPC kinetics and also for the interpretation and conduction of future clinical studies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available