Myosin Binding Protein-C (MyBP-C) is a multi-domain protein located on the thick filaments of striated muscle. Mutations in the gene encoding the cardiac isoform of the protein, cMyBP-C, are known to cause hypertrophic cardiomyopathy (HCM). cMyBP-C is composed of 11 globular domains, eight of which have homology to Igl, the other three to fibronectin III. The N-terminal region of the protein has been assigned a role in the regulation of muscle contraction through its interaction with the Subfragment-2 (S2) portion of myosin.