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Title: Functionalised Flagellar Nanotubes
Author: Woods, Richard David
ISNI:       0000 0001 3572 3995
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2007
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This project investigated the Potential for using the bacterial flagellar filament as a functionalized protein nanotube. A varied approach to flagellin engineering was taken using the flagella of E. coli and R. sphaeroides, with engineered flagellar flilaments from E. coli proving successful. A variety ofepitopes were inserted into the E. coli flagellin, resulting in filaments which displayed adhesive peptides or cleaveable sites. His-tagged filaments allowed the binding of metal ions and nanoparticles, and the biotin-avidin system was empl~yed to allow more specific binding of ligands. Cys-tagged filaments were 'made to order' for a collaborator to allow improved binding of gold to flagellar filaments. The TEV protease recognition site was used to allow specific cleavage of the surface displayed portion of the flagellin within the intact filament structure. Other proteases allowed further digestion ofthis surface displayed region. By combining the adhesive epitope with flanking TEV protease sites selective excision of the tag was possible, allowing the potential for directed whole filament removal from a surface or scaffold in applications such as nanolithography. An in trans expression system was developed to allow assembly of different flagellins into the same filament, with expression levels varied to result in specific ratios of each functionalised monomer. This allowed the creation of bi-functional protein nanotubes, which could bind more than one ligand.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available