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Title: An Investigation Into MHC-Determined Resistance to Marek's Disease
Author: Crouch, Kathryn Mary
ISNI:       0000 0001 3398 5895
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2007
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Cell-surface expression ofmajor histocompatibility complex (MHC) class I was investigated in nine chicken lines using a quantitative assay. Levels were 3-4 fold higher on peripheral blood mononuclear cells (PBMC) from the Marek's disease (MD)-susceptible line P2a than from resistant line N. The ratio of class I on PBMC to red blood cells was greater in haplotypes expressing lower levels of class I, indicating that class I complexes may be less stable in these haplotypes. Infection oflines P2a and N with MDV confirmed that these lines are respectively susceptible and resistant. However, an FI cross of these lines was more susceptible than predicted from the literature. Blood viral load decreased in line N at 7-10 dpi, indicating an antiviral response in this line that is ineffective in other lines examined. These experiments indicated the involvement of multiple genetic loci in resistance to MD in this model. An attempt to map loci associated with resistance to MD using a candidate approach in an F2 population was inconclusive due to a breeding error. A flow cytometry-based assay was developed to identify MDV-infected cells from infected birds. Cytolytically infected cells expressed higher class I levels than uninfected cells in vivo despite observations to the contrary in vitro. An attempt to determine if there was any difference in the degree of up-regulation between lines P2a and N was inconclusive.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available