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Title: Magnetic resonance imaging of cellular and molecular events in inflammation
Author: Reynolds, Peter Robert
ISNI:       0000 0001 3514 9970
Awarding Body: Imperial College London (University of London)
Current Institution: Imperial College London
Date of Award: 2007
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Imaging leucocyte trafficking is a major goal in inflammation research, since one ofthe key features of the immune system is cell migration. Ultrasmall iron oxide nanoparticles (USPIO) are iron-based T2-enhancing magnetic resonance (MR) imaging contrast agents, which are a different class ofcontrast agent compared to the more traditional, clinically-established Tl-enhancing agents such as GadoliniumDTPA. The work presented in this project investigates different methods using both these classes ofcontrast agent for imaging different aspects ofinflammation at cellular and molecular levels. The uptake and MR imaging ofUSPIO and Gadolinium-DTPA by leucocytes in-vitro and in-vivo has been investigated. Activated endothelium is a proven surrogate for inflammatory sites. E-selectin is an adhesion molecule which is luminally expressed by activated endothelium early-on in the inflammatory process. This work has tested the hypothesis that activated endothelium can be imaged using magnetic resonance. USPIO have been conjugated with a monoclonal antibody targeting expressed Eselectin, and tested in-vitro and in-vivo using a murine model ofoxazolone-induced contact hypersensitivity inflammation in the ear. The majority ofMR studies in this project were conducted at a high MR field strengths of9.4T. The results indicate that MR imaging ofleucocyte migration and ofmolecular markers of activated endothelium are feasible. However there are limitations on the degree of success, which are presented and discussed. Targeted and cell specific imaging ofinflammatory lesions has the potential to be important investigative tool.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available