Use this URL to cite or link to this record in EThOS: | https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.487251 |
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Title: | Mechanisms of secretion at the immunological synapse | ||||
Author: | Holt, Oliver J. |
ISNI:
0000 0001 3580 9684
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Awarding Body: | University of Oxford | ||||
Current Institution: | University of Oxford | ||||
Date of Award: | 2007 | ||||
Availability of Full Text: |
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Abstract: | |||||
Cytotoxic T lymphocytes (CTLs) and Natural Killer (NK) cells kill virally infected
and tumourogenic cells. CTLs and NK cells engage directly with their 'target' cell,
polarise their secretory machinery towards the point of contact, or immunological
synapse (IS), and release a volley of killing agents that induce target cell apoptosis.
The exocytosis oflytic granules (specialised secretory lysosomes) at the IS is highly
regulated, and crucial for the delivery of apoptotic factors to the target cell. The
molecular components that regulate and mediate this process are poorly characterised
in eTLs and NK cells. I have shown that a Rab27a effector protein, Synaptotagminlike
protein 2a, is important for the killing process. I have also investigated the
expression, localisation and interactions of SNARE C§.oluble NSF ~ttachment
receptor) proteins within eTLs and NK cells.
Fas Ligand (FasL) is one of the lytic agents stored within lytic granules. The sorting
of FasL direct to lytic granules relies upon the Src tyrosine kinase Fgr, and may
require other binding partners. I have characterised the binding of SH3 domains from
three different proteins, including Fgr, with the cytoplasmic tail of FasL to help
establish their roles in the sorting of FasL to lytic granules.
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Supervisor: | Not available | Sponsor: | Not available | ||
Qualification Name: | Thesis (Ph.D.) | Qualification Level: | Doctoral | ||
EThOS ID: | uk.bl.ethos.487251 | DOI: | Not available | ||
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