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Title: Mechanisms regulating lymph node organogenesis
Author: White, Andrea Jane
ISNI:       0000 0001 3566 8084
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2006
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The fonnation of organised microenvironments within lymph nodes(LN) occurs during development and is essential to support effective immune responses. Organogenesis ofLNs requires interactions between LTI* expressing mesenchymal stromal organiser cells and LTaJI32 expressing hematopoietic LTi cells. This activates the NF-KB signalling pathway, inducing expression of chemokines and adhesion molecules that are essential for the continued recruitment and retention ofLTi cells, and ultimately the recruitment oflymphocytes to the iLN. However the cellular and molecular mechanisms ofLN development are still unclear. This study has developed novel techniques to study iLN development, including the isolation ofiLN from embryos, the generation of LTi cells in vitro, and the reaggregation and transplantation ofdifferent cellular subsets to study their interactions in vivo. We have identified a programme ofstromal cell development, related this to the role of LTi cells in iLN organogenesis and identified LT independent and LT dependent phases ofiLN development. Finally a potential precursor/product relationship between fetal LTi cells and adult CD4+CD3- cells has also been highlighted as a result ofphenotypic analysis and precursor-product relationships. To conclude, this thesis contains a detailed study into the cellular requirements and molecular mechanisms involved in iLN organogenesis.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available