Use this URL to cite or link to this record in EThOS:
Title: RNA-degradation in the nematode worm C. elegans
Author: Bowen, Caroline Sarah
ISNI:       0000 0004 2667 998X
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 2007
Availability of Full Text:
Full text unavailable from EThOS.
Please contact the current institution’s library for further details.
RNA turnover is necessary to ensure correct levels of gene expression, through posttranscriptional gene regulation and to remove aberrant mRNA transcripts, thereby maintaining RNA quality. Here we examine processes of RNA degradation in C. elegans, based on a functional investigation of the C. elegans components of RNA degradation pathways, focusing specifically on the exosome components, identified from sequence similarity to other organisms. RNAi and available mutants have been used to show that disruption of the exosome complex, through the targeted degradation of individual components, results in worms with severe developmental problems including slowed growth rates and developmental defects. Full length fluorescent tagged exosome components were generated, and have shown expression to be largely confined to the nuclei of all cells, including the germ line. Phenotypes associated with depletion of the exosome components are attributed to effects on rRNA processing. When exosome components are silenced, there is a failure of the degradation of specific cleaved rRNA precursors, which in tum interferes with ribosome function and subsequently protein synthesis. The investigation of the nature of these rRNA processing defects, as well as the role of the exosome in other RNA pathways such as RNAi and NMD, are presented. Finally genes involved in ribosome biogenesis are investigated, depletion of which has significant effects on development, causing. germ line tumours similar to those seen for exosome depletion.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available