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Title: Characterisation of the Murine Expression of Three PAQR Subtypes with Putative Membrane Progesterone Receptor Activity
Author: Patel, Zabeen
ISNI:       0000 0001 3476 4349
Awarding Body: Imperial College London (University of London)
Current Institution: Imperial College London
Date of Award: 2007
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Background In 2003, the: fish membrane progestin receptor (mPR) was identified as a putative mediator of rapid, non-genomic progesterone action 1)Subsequent biointormatic analysis revealed homologues of this receptor in various vertebrate species including three in the human and mouse: mouse mPR a, b and y. 2)These subtypes Were: later to be shown to have distinct expression profiles in various human gestational tissues, and were also found to be a part of a larger 1amily of proteins, called the Progestin and Adiponectin Receptors (PAQR). and named PAQR. VII, VIII & V, respectively (3)The aim of the project was to elucidate the in vivo role of thll three PAQR members with putative, non-genomic, mPR activity, via expression profiling at the mRNA and protein levels, within various murine models. Results to Date There was no significant difference in expression of each PAQR subtype. in murine ovary and uterus, across the oestrus cycle, nor in tissues that were null for the nuclear progesterone receptor (nPR), compared to wild-type controls. This suggests that the expression of murine putative mPRs are not regulated by endogenous levels of progesterone, nor by the genomic progesterone signaling pathway. An anti-murine PAQR vn antibody was generared in order to characterize the cellular distribution of this subtype. The antibody has been validated to be specific for its target, using various controls. and has been shown to function in preliminary immunohistochemistical assays. ~urther 89says are being carried cut to determine the exact pattern of PAQR VII expression under different physiological conditions. References 1. Zhu Y, Rice CD, Pang Y, Pace M, TboII1liS P (2003) Cloning, expression, and charllC~rizatjon of a membr.ltlc progestin receptor and cvi(,lI;ncc it is an incermedi:uy in mcioticmaturation offish oocytes. P/'DC Natl Acad Sei USA 100:2231--6 2. Zhu Y, Bond 1. Thumas P (2003) fdcntification, classification, Qnd partial characterization of l:ents in humans and other vertebrates homologous to a fish membrane progestin receptor. Pr(J~ Nail ..teat!Sci USA 100:2237-42 3. f'emandc:s MS, Pierron V, Michalovich D. A~11���¢ S, Thomtol\ S, Peltoke:to H, Lam EW-F, Gellersen B, Huhraniemi r, Allcn J and Bruscns J (2005) ~gulaIcd c:xpn:ssion of putative membrane progestin receptor homologues in humzsn cndometriwn and gesr.arianaJ tj.~~~ ���� .1 ���£nd(]crintllfWV I fi7:K9-1 01
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available