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Title: Induction of antioxidant response element-linked gene expression by dietary isothiocyanates
Author: Imbasi, Precious Ayebaifemi
ISNI:       0000 0001 3586 400X
Awarding Body: University of Essex
Current Institution: University of Essex
Date of Award: 2008
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Oxidative damage to DNA and proteins contributes to mutation and cancer risk. A further source of DNA and protein damage is glycation by reactive endogenous dicarbonyls - particularly methylglyoxal and glyoxal. Cellular protection against oxidative damage involves up regulation of enzymes controlled transcriptionally by transcription factor nrf2 interacting with an antioxidant response element (ARE) in the promoter. Dietary isothiocyanates (ITCs) enhanced enzymatic components of the antioxidant defence by increasing the ARE-linked gene expression. This may also increase protection against dicarbonyl glycation - provided by glyoxalase I (GLO I) and aldoketo reductases. I assessed the potency of dietary ITCs as inducers of ARE -linked gene expression, related cytoprotective effects and inhibition of tumour cell growth in vitro. ARE-linked gene expression was assessed by enzymatic activity assay and immunoblotting, protection against glycation and oxidative stress by assay of dicarbonyls and reactive oxygen species (ROS), glycation linked mutation by green fluorescent protein linked reporting vectors and cytotoxicity to tumour cells by counting viable cell number.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available