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Title: Physiology and Crossbridge mechanism of bony fish muscle
Author: Eakins, Felicity Elizabeth Veronica
ISNI:       0000 0001 3436 8661
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2008
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Muscle contraction is brought about by the interaction of the proteins actin and myosin. Xray diffraction is a useful tool for gaining information about this process. This thesis reports on the effect of sarcomere length on the X-ray diffraction patterns from active and rigor bony fish muscle. During contraction, sarcomere I.ength changes are known to slow the development of tension (Cecchi et al., 1991) and effect X-ray reflection intensities, (Elliott et al., 1963). Up until now the size of the contractile sarcomere length change in these muscles was unknown and its effect on the tension and X-ray intensities had been neglected (Harford and Squire, 1992). Previously, a time lag was observed between the intensity changes of . the first two major equatorial X-ray reflections (A(10) and A(11�». This led to a hypothesis that the low and high force attached crossbridge states are structurally distinct (Harford and Squire, 1992). In this project, a sarcomere length measurement and control system was developed. The contractile performance of the bony fish muscles was also improved. For the first time, the sarcomere length change during contraction of whole Plaice fin muscle was measured (a reduction of (3.10�±0.06)% per sarcomere) and the system could halve this change. This sarcomere length control was found to significantly increase the rate of tension development. X-ray data also showed a reduction of over half in the lag between the two intensity changes (A(10) and A(11�», providing less clear evidence that the two crossbridge states are structurally different, a finding closer to that seen in frog muscle (Cecchi et al., 1991). Using two pre-existing X-ray datasets, the effect of initial sarcomere length on the state induced in rigor bony fish muscle was also investigated. Evidence from the intensity distributions on the actin layer-lines and from electron density maps of the muscle crosssection, (Harford et al., 1994), suggested that in rigor muscles with a longer initial sarcomere length than the conventional 2.2lJm, a different state was induced in the specimens. This was characterised by a smaller tropomyosin shift, a different crossbridge labelling pattern and a different average head shape, possibly closer to that seen in active muscle.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available