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Title: Calcium imaging in rat retinal arterioles
Author: Tumelty, James Martin
ISNI:       0000 0001 3537 692X
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2007
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With the use ofconfocal microscope, the work presented in this thesis has for the first time identified spontaneous Ca2+sparks and Ca2+oscillations in intact retinal arteriole smooth muscle cells. Ca2+sparks may act as building blocks that can summate to interventions that modified ci+ sparks also modified Ca2+oscillations. The mechanisms include, release of Ca2+from ryanodine sensitive stores, the sarcoplasmic reticulum Ca2+-ATPase and Ca2+influx from the extracellular space. However, the influx of Ca2+is not tightly correlated to spontaneous Ca2+release. The effects of the vasoconstrictor, endothelin-l on subcellular Ca2+signals was examined and was found to evoke Ca2+ oscillations. Further studies showed that endothelin-l' increases the frequency ofCa2+ oscillations by binding to the endothelin-A receptor with the subsequent release ofCa2+ from IP3 sensitive and ryanodine sensitive stores. A further increase in the frequency of Ca2+oscillations is seen with the inhibition ofvoltage gated K+ channels, suggesting these channels act to suppress membrane excitability.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available