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Title: Identification of tumour-specific T-cells in colorectal cancer patients
Author: Robinson, Jonathan Matthew
ISNI:       0000 0001 3528 5139
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2006
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The ability to induce an immune response to a tumour is an attractive approach to cancer treatment, particularly when used in combination with surgery and chemotherapy or radiotherapy. In theory the use of such immunological techniques should be possible to help treat colorec~ cancer. Immunotherapy has shown encouraging results in the treatment of other cancers, namely malignant melanoma and renal cell c¥cinoma: The ability of these techniques to aid the treatment of advanced colorectal cancer has so far proved disappointing, although some encouragement has been observed. It has recently become possible to identify CD8+ T-cells specific for particular antigens using MHC class I tetramer staining. Carcinoembryonic antigen (CEA) is one such tumo~ antigen that is normally only expressed during embryonic development and not by adult tissues. However, in patients with colorectal cancer, CEA is over expressed and is therefore considered to be a tumour-specific antigen (TAA). T-cells specific for an epitope of CEA (CAP-I) can be identified using CAP-I loaded MHC class I tetramer molecules. The aim of this project was to assess the number and frequency of CEA specific CD8+ T-cells in colorectal cancer patients using this tetramer staining'protocol; in particular during the different treatments they receive prior to surgery, namely radiotherapy and ' chemotherapy. Previous methods to study these 'cells required large volumes of blood not suitable for patient studies. A novel and enhanced technique for the generation of antigen specific T-cells was employed which only required small volumes of blood . ideal for repeated patient testing. Cells recovered from the peripheral blood of healthy controls were cultured and observed for the proliferation of CAP-I specific T-cells, which were detected by tetramer staining, replicating the results of previous studies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available