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Title: Development of a tissue engineered human oral mucosal model for the assessment of the biocompatibility of resin-based dental materials
Author: Moharamzadeh, Keyvan
ISNI:       0000 0001 3413 7871
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2006
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Restorative materials and oral health care products come into direct contact with oral mucosa. Components of dental composite resins can be released into the oral cavity and can cause adverse reactions. To obtain an accurate risk assessment, the in vitro test model must reflect the clinical situation as closely as possible. Therefore, the aim of this study was to develop a three-dimensional tissue engineered human oral mucosal model to assess the biocompatibility of resin-based dental materials. In this study in vitro biocompatibility of resin-based dental materials was assessed using a variety of laboratory techniques including cytotoxicity tests on different monolayer cultures of epithelial cells, chemical analysis of components released. from different composite resin systems, and finally a tissue-engineered human oral mucosal model was developed, characterised and examined for biological assessment of resin-based dental materials. The results showed that dental resin monomers were toxic to the epithelial cells and the monomer TEGDMA was the major component released from composite reins in quantifiable amounts and may be sufficient to cause an adverse reaction. The type of extraction media and the time of analysis had a significant effect on the detection of monomer released from composite resins due to protein binding. A welldifferentiated and highly reproducible full-thickness oral mucosal model was developed and characterised that has the potential to be used for mucotoxicity and biocompatibility assessment of dental materials. Exposure to high-TEGDMA containing composite resins caused a significant mucotoxicity and increased the amount of IL-I J3 released from the oral mucosal model. The 3-D tissue engineered human oral mucosal model is believed to be more clinically relevant than monolayer culture of epithelial cells and the results obtained from multiple-endpoint analysis of the oral mucosal model were more coherent.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available