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Title: Applying forces to human osteoblasts and mesenchymal stem cells using a novel magnetic particle technique
Author: Kirkham, Glen
ISNI:       0000 0001 3600 380X
Awarding Body: Keele University
Current Institution: Keele University
Date of Award: 2007
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The study and application ofmechanical forces to the cells ofthe body, has been proposed as a means of engineering replacement tissues. In order to investigate the role of force in .' any cell type, it is necessary to design and contract an artificial means of applying force in vitro and in vivo. The use of such methods in the study of the cellular responses of osteoblasts has been carried out for a number of years. In contrast, the roje of mechanical forces in the regUlation of Mesenchymal Stem Cells (MSCs) is currently unknown. A ~eries of laboratory-based studies were used to investigate some of the cellul~ processes involved in the regula~ion of MSCs and osteoblasts, in'response to a novel method of mechanical force application. This involved the use ofmagnetic particles coated with Arg- '. Gly-Asp, in order to target their binding to cell surface integrins. Magnetic fields were then applied using two magnetic field application systems, designed and constructed during the course ofthis study. There were, therefore, two principal aims ofthis thesis. 1) to assist in the design and construction of the two field application systems, and to establish the basic biological protocols for their effective use. 2) to investigate any cellular responses of MSCs and osteoblasts to this method of force application. Both cell types demonstrated significant membrane hyperpolarisation responses after force application, mediated by both . large capacitance BK potassium channels, and calcium release from intracellular stores (not extracellular calcium influx). No changes in cell signalling and the levels osteogenic genes were detected, although this was likely due to the configuration of the particle technique. Finally, the movement ofparticles during force application was tracked and this showed stable particle adhesion, with a possible small degree ofplastic deformation, after the application ofa magnetic field.
Supervisor: Not available Sponsor: Not available
Qualification Name: Keele University, 2007 Qualification Level: Doctoral
EThOS ID:  DOI: Not available