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Title: Fluorigenic enzyme assays using long-wavelength substrates
Author: Sarpara, Ghulam Hussain
ISNI:       0000 0001 3553 3278
Awarding Body: Loughborough University
Current Institution: Loughborough University
Date of Award: 2000
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Investigations into the use of long-wavelength fluorigenic substrates, which are non- or weakly fluorescent but are converted by an appropriate enzyme to a highly fluorescent product, have been carried out in order to determine several different enzymes and their inhibitors. Certain nonfluorescent compounds based on long-wavelength xanthenes and oxazines dyes were synthesised. Amongst these, naphthofluorescein diphosphate and naphthofluorescein mono-phosphate has been demonstrated to be new fluorigenic substrates for alkaline phosphatase. The hydrolysis product of these substrates can be used for trace analyses using diode laser based fluorescence detection.
Supervisor: Not available Sponsor: Ministry of Education, Pakistan
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Organic chemistry