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Title: Chloroplast gene expression during leaf development
Author: Silverthorne, Jane
ISNI:       0000 0001 3411 7491
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 1980
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The growth and development of the primary leaf of spinach (Spinacia oleracea) have been characterized with respect to both physical and biochemical parameters. Excised primary leaves incorporate [^”*s] methionine into a number of chloroplast polypeptide The ratio of incorporation of isotope into two chloroplast polypeptides, the large subunit of Fraction I protein and a thylakoid polypeptide (peak D), decreases during leaf development in whole leaves. This changing pattern of incorporation is also observed in isolated chloroplasts where these two polypeptides cure the major products of protein synthesis. Chloroplast RNA prepared from developing leaves was translated in a cell-free protein synthesizing system prepared from rabbit reticulocytes to yield full-length large subunit and peak D polypeptides. The fidelity of translation of these two polypeptides was checked by partial protease digestion. Changes in the synthesis of the large subunit of Fraction I protein and peak D in developing leaves are reflected in changes in the amount of translatable mRNA for these two polypeptides. These findings suggest that the expression of chloroplast genes during development is at least partially controlled at the transcriptional level.
Supervisor: Not available Sponsor: Strategic Research Council
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QA Mathematics