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Title: Immunological markers in hairy-cell leukaemia
Author: Burns, Gordon Frood
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 1979
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The dissertation consists of six chapters. Chapter 1 presents a brief introduction on immunological markers. Chapter 2 describes the discovery of a previously unrecognised marker in hairy-cell leukaemia (HCL), a receptor for IgM. The rosette method used to reveal this receptor, and the conditions affecting rosette formation are described. A kinetic study of this receptor is used to provide a measure of membrane turnover in HCL. The distribution of the IgM receptor among normal and malignant haemic cells is investigated. Chapter 3 considers a variety of other immunological markers in HCL. Previous controversy in the literature concerning the presence of a receptor for complement on hairy cells is resolved and new information is provided about the presence of other markers on hairy cells. Chapter 4 presents experiments designed to investigate conflicting evidence regarding the nature of the hairy cell. Monocytic properties are measured and weighed against features of hairy cells which align them with the B-lymphocyte series. The presence of intrinsic surface immunoglobulin (Sig) is used as evidence for the B-cell nature of HCL. The results obtained showing only IgG on the surface of some cases of HCL, and multiple heavy chain isotypes on others, provide a solution to the confused literature regarding the presence of SIg on hairy cells. Two unusual cases of immunoproliferative diseases are extensively investigated in Chapter 5 in order to demonstrate some of the problems associated with immunological marker studies. Chapter 6 reviews the thesis and draws conclusions about the nosology of HCL. A possible position for HCL in schemes of B-cell differentiation is given. Several papers of unconnected work submitted during the period of research for the dissertation are also included for consideration by the Examiners.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral