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Title: Cell proliferation in human oral mucosa
Author: Warnakulasuriya, Kasturi Arachchi Ariya Saman
ISNI:       0000 0001 3563 4511
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1976
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Studies of cell proliferation and migration - cell kinetics - have been described extensively on animal tissues and to a more limited extent in human tissues. These reported studies are reviewed and the deficiencies in available knowledge are highlighted. One of the main difficulties in studying the kinetics of human tissues is that the most widely used technique in animal work, namely parenteral injection of radioactive isotopes to label specific cells, is not generally applicable in man. The initial work in the present study was concerned with investigation of techniques for quantitating cell kinetics and the development of an in vitro radioactive labelling technique suitable for use with human tissues. The reliability of this technique was evaluated in the animal study where the in vitro labelling was shown to give comparable results to the classical in vivo labelling techniques. The in vitro radioactive labelling technique was then used on biopsies from normal cheek mucosa obtained at six times of the day. This allowed diurnal variations in cell kinetic parameters to be quantitated. In conjunction with this investigation a stereologic study of the epithelium was undertaken. The cell kinetic and stereologic techniques were next used to study eight cases of leukoplakia of cheek, a clinical disorder characterised by keratosis. The findings in the leukoplakia lesions were contrasted with those in biopsies from clinically normal mucosa in the same individuals. Epithelial cell production was found to be increased although the viable cellular layers of the epithelium were atrophic in the leukoplakias. The control biopsies, although obtained from clinically normal mucosa, were shown to exhibit several abnormalities. The degree of increased cell producation in leukoplakia in these control specimens was shown to have a positive correlation with the severity of epithelial atypia. The further applications of kinetic studies and epithelial morphometry are outlined and the difficulties encountered in the use of in vitro radioactive labelling techniques and other morphometric methods in the study of cell proliferation are discussed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available