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Title: Physiology of certain sterile septate fungi associated with Ectomycorrhizae of Pinus sylvestris
Author: McKeogh, Pamela Annette
ISNI:       0000 0001 3625 2400
Awarding Body: University of London
Current Institution: Royal Holloway, University of London
Date of Award: 1976
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Ninety-one root isolates were isolated from the sctomycorrhizal roots of Pinus sylvestris. Boletus variegatus 1 and Boletus luteus were isolated from sporophores. Electron micrographs showed the dolipcre septum to be present in Boletus lutauo, but a simple pore occurred in the septum of root isolate M1. Boletus lutaus and some root isolates caused the dichotomy of the secondary laterals of Pinus sylvestris seedlings grown on Pachlewski agar slopes, 10-6M indole-3-ecetic acid had a similar affect. Attempts to synthesize ectomycorrhizae in vitro between Pinus sylvestris and root isolatee, Boletus variagatus 1 or Boletus luteus were of limited success. Several established techniques and modifications of them were used. However many root isolates, else Boletus variegatus 1, were significantly stimulated by live excised tomato roots, this being a characteristic of ectcmyccrrhizsl fungi, although Boletus variegatus 1 and the root isolate m13 reacted differently to exudates, extract and homogeneities of excised tomato and pine seedling roots. Specific M13 growth stimulatory fractions of excised tomato and pine seedling root preparations were obtained by paper chromatography using two different solvent systems. There ware no specific Boletus variegatus 1 growth stimulatory fractions. A number of organic compounds were positively identified by paper chromatography to be present in the excised tomato root preparations. Riboflavin had similar fractions as the M13 growth stimulator(s) in both solvent systems. The identity of these stimulatory spots as riboflavin was confirmed by the use of the Mass Spectrometer. Also its effect on the growth of M13 was determined. The nutritional requirements of mI 3 were similar to these of several unnamed ectomycorrhizal fungi, but differed from the requirements of certain named ectomycorrhizal fungi, for example Boletus variegatus.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Botany