Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.463097
Title: Trace element studies using activation analysis
Author: Leslie, Alistair Charles Day
ISNI:       0000 0001 3608 3483
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1975
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Abstract:
This thesis describes the estimation of elements at trace levels in biological samples, mainly human, by the technique of thermal neutron activation analysis. The work falls into three main parts. In the first part the history, background, principles and details of the activation analysis technique are described; in the second part details of the methods used and their modification or development are described; and in the third part the applications made to trace element studies are described. In neutron activation analysis the induced radioactivity of an element enables its concentration in a sample to be assessed. The advantages of this technique made it a suitable choice for the determination of the low levels of trace elements in biological tissue. A search of the literature for neutron activation analysis techniques reveals that analysis methods for many of the elements have been described already. Methods found suitable for copper, mercury and cadmium analyses are used as described. Methods described for arsenic, gold, manganese and zinc analyses are modified to improve them and make them more suitable. For selenium and barium analyses it is necessary to develop new methods. The development of these methods is described and discussed. All methods are then applied to the trace element analyses described in the following section. The trace element studies carried out fall into three fields of research: (1) A study of the role in tissue of some trace elements, (2) A study into environmental uptake by man, and clinical application of the analysis technique, (3) A study of specimens of historical interest. (1) The question of whether an element is essential or nonessential to a tissue may be indicated by distribution studies. Using this as a basic criterion, manganese, cadmium and selenium are investigated in the major tissues of the human body. Manganese is also investigated in Rhesus monkey tissue and levels of human and monkey tissue compared. Manganese and selenium are established as essential and cadmium is found to be nonessential. The role of barium in the human lung is investigated and it is shown to be nonessential and to accumulate in the broncopulmonary lymph nodes. The variation with time of copper levels in the nail and skin of an individual is investigated and shown to be held within a narrower range than that found in the whole population. (2) A study is made of the occupational uptake of mercury in dental groups. Workers who were industrially exposed to mercury, arsenic and copper are also examined. A high uptake is found in all these groups. A feature, that is found common to all groups, is that a surprisingly high amount of absorption could be tolerated in many cases, without the associated occurrence of poisoning symptoms. Thus, the value of the analysis as a means of detecting a potential health hazard is demonstrated. Arsenic analysis of hair, nail and urine is shown to be of use in monitoring a case of known arsenic poisoning. Head hair analysis enables, very elegantly, a record of previous uptake to be found. Analysis for arsenic and mercury is able to be extended into further clinical application and is proved to be useful. Zinc levels in salivary glands are investigated to see what part zinc played in their function. Lower zinc levels than in other soft tissue are found, indicating that here fewer zinc associated metabolic processes are involved. A possible association between zinc in saliva and the labial gland is found. (3) The value of the activation technique for the examination of small historical specimens is demonstrated by the analysis undertaken of mercury in head hair from Robert Burns and of arsenic in head hair from Napoleon Bonaparte. It is thus shown that Burns may well have been suffering from mercury poisoning, but the evidence is not conclusive. It is found that there is much evidence to suggest that Napoleon was suffering from consistent arsenic poisoning during 1816. In conclusion, the technique of neutron activation analysis is developed and applied to biological materials. It allows simple, but precise, investigation to be made into trace element levels in both living subjects and in other tissues where only small samples are available.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.463097  DOI: Not available
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