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Title: Studies on the synthesis of the small subunit of Fraction I protein
Author: Highfield, Peter Edmund
ISNI:       0000 0001 3556 2909
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 1978
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The small subunit of Fraction I protein from pea leaves has been synthesised in vitro by translation of poly(A)- containing RNA , obtained from pea shoot polysomes, in a cell- free protein-synthesising system prepared from wheat-germ. The products of this translation were characterised by gel electrophoretic and immunological techniques. It was found that the small subunit of Fraction I protein was synthesised as a precursor of 20,000 molecular weight; this precursor has been called P20. In the presence of isolated, intact chloroplasts, P20 is processed to the small subunit. The small subunit so produced is resistant to trypsin-digestion and so is assumed to have entered the chloroplasts. The processing activity is apparently associated with the chloroplast envelope. A mechanism for this processing and transport is proposed and is discussed in the light of other models currently available for the transport of proteins across membranes.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QD Chemistry ; QK Botany