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Title: The role of cell interactions in lymphocyte circulation
Author: Davies, Miles David Joseph
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1978
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It has been known for some time that filtration of a heterogeneous population of lymphocytes through a nylon wool column will produce an cluate containing almost exclusively T lineage lymphocytes. Results presented in this thesis show that filtration of thymus lymphocytes through nylon wool producer, a subpopulation which exhibits both a greater mutual adhesiveness and a temporary difference in localisation patterns when injected back into syngeneic recipients as compared to the unfiltered population. Further analysis of the adhesiveness and localisation patterns of filtered and unfiltered T lymphocytes derived from lymph nodes was used in an attempt to support the hypothesis that the in vivo localisation characteristics of a lymphoid cell can be predicted from its in vivo adhesiveness to like cells. The data, however, was not compatible with this concept which was rejected as being too simple. It was found that the localisation of particular radioactively labelled lymphoid cells could be disrupted by the simultaneous injection of a large number of unlabelled lymphoid cells of a homogeneous lineage. This effect is not thought to be the result of physical obstruction by the extra cells but rather due to substances released by the unlabelled cells which alter the interaction of the labelled cells with resicent and circulating cells within the lymphois system. The presence of substances released by lymphocytes which can modulate the interactions between lymphocytes of the opposite lineage has been previously reported. The effect of these substances, interaction modulation factors (IMFs), on the localisation of injected labelled lymphoid cells was studied and the results suggested that the effects seen after treatment of injected labelled cells with large numbers of unlabelled cells could possibly be attributed to the release of large amounts of IMFs by the latter cell type. The stages of lymphocyte circulation and their possible control mechanisms are discussed in depth but none can adequately explain the overall positioning patterns that are displayed by lymphoid cells. The action of IMFs in vivo is put forward as an overall control mechanism of lymphocyte circulation and is compared with other theories which have been suggested to explain the specific sorting-out of cells in embryonic and morphogenetic systems.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available