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Title: The effects of interferon and double-stranded RNA
Author: Cooper, Jonathan Alstead
ISNI:       0000 0001 3562 2633
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 1976
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1. The effects of dsRNA on interferon-treated and control cells, and on various cell-free systems, have been characterised, and discussed in relation to theories on the role of dsRNA in the control of cell functions. 2. The double-stranded homopolymer, poly rl.rC, used for most of these experiments, was characterised in terms of its resistance to ribonucleases and its behaviour on cellulose column chromatography. 3. The effects of various homopolymers on protein synthesis in the reticulocyte lysate were studied and related to their published effects on intact cells. The apparent requirement for high molecular weight is discussed in relation to measurements of structural stability. 4. The cytotoxic effect of dsRNA on interferon-treated L929 cells was characterised, and a proportion of the cells resistant to the toxicity was found to exist. No evidence for a specific biochemical trigger for toxicity could be found. 5. A cell-free protein synthesising system from monolayer grown L929 cells was developed and characterised. 6. An assay for an interferon and dsRNA dependent inhibitor of protein synthesis was developed using reticulocyte lysates. Interferon-treated cell cytoplasm was capable of forming an inhibitor of protein synthesis when incubated with dsRNA and ATP, which inhibited a specific step in protein synthesis initiation. The relevance of this inhibitor to changes in protein phosphorylation is discussed. 7. There was no evidence that the inhibitor is generated when interferon- treated cells are exposed to dsRNA, so the inhibitor may not be involved in the cytotoxic effect.
Supervisor: Not available Sponsor: Cancer Research Campaign
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QP Physiology ; QR Microbiology