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Title: A Cytochemical and Fine Structural Study of the Root Tips of Zea Mays with Particular Reference to the Geotropic Response
Author: Al-Azzawi, M. J.
ISNI:       0000 0001 3405 1015
Awarding Body: University of Sussex
Current Institution: University of Sussex
Date of Award: 1978
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The correlation between the structure and function of cells may be studied in a number of ways. Some of these methods have been used in this thesis in an attempt to study the elongation zone of geotropically stimulated maize root tips and wheat internodes. Using a cytochemical technique, adenyl cyclase activity has been investigated in the cells of maize root tips and other tissues using a specific substrate developed originally for mammalian tissues. Heavy deposits were found in the plasma membrane, endoplasmic reticulum and nuclear membrane, and the findings are discussed in relation to the reported localization of adenyl cyclase in animal and plant cells. As with adenyl cyclase, no distinct changes were found in the localization of ATPase and peroxidase activities in relation to the geotropic response. The effect of aldehyde fixation of excised roots and of subcellular fractions of maize were studied in relation to the preservation of ATP-ase and peroxidase activities. Total peroxidase was little affected by either formaldehyde or glutaraldehyde whereas ATP-ase showed a considerable loss of activity, particularly with glutaraldehyde. The activity remaining after fixation was dependent on both the concentration of fixative and the pH of fixation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available