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Title: The role of lymphocytes in the pathogenisis of diabetic retinopathy
Author: MacKinnon, Jane R.
ISNI:       0000 0001 3615 6099
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2006
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Methods: Fresh blood samples were collected from 69 age-matched subjects consisting of 45 patients with diabetes (with or without retinopathy) and 24 controls.  In vitro hyperglycaemic incubations were performed to detect any changes in surface expression of markers of activation (CD69 and CD25 / IL-2 receptor).  Patient and control samples underwent flow cytometry for the same markers of activation and also 4 adhesion molecules: LFA1, VLA4, ICAM-1and L-selectin. Results: In vitro hyperglycaemic incubations did not induce a significant change in CD69 or CD25 levels.  Significantly reduced L-selectin expression was found on lymphocytes from patients with diabetes compared to controls (p=0.004).  The lowest levels of expression were found in those with proliferative diabetic retinopathy (p=0.001).  There were no significant differences in surface expression of the markers of activation and the other adhesion molecules studied.  Increased nuclear production of L-selectin was indicated by the finding of significantly higher mRNA levels (p=0.007) in patients with DR than in those with no retinopathy.  Similarly, serum L-selectin levels were significantly higher (p=0.04) in those with DR compared to controls.  Lymphocyte adhesion relative to control was essentially unchanged for diabetic patients with no retinopathy but was markedly increased for those with retinopathy (p=0.0001). Conclusion: Lymphocyte activation, reduced surface LO-selectin, increased circulating and nuclear L-selectin levels, and a corresponding increase in adhesion to endothelial cells is evident in people with diabetic retinopathy.  This suggests a role for lymphocyte activation in the pathogenesis of diabetic retinopathy.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available