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Title: Microspheres as carrier systems
Author: Muzerelle, Mathilde
ISNI:       0000 0001 3437 8659
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 2005
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Micron and submicron microspheres were successfully synthesised and their cytotoxicity toward various cell lines determined.  The influence of parameters such as incubation time, concentration and size of the microspheres on cellular cytotoxicity was evaluated and showed overall little cytotoxicity. The cellular uptake of these beads was studied using carboxyfluorescein labelled beads and it showed efficient uptake at low concentrations, and with short incubation times. Peptides and enhanced green fluorescent protein were covalently coupled to the microspheres and shown by flow cytometry and fluorescent confocal microscopy that uptake by the cells was fast and efficient proving the potential of the crosslinked aminomethyl polystyrene microspheres as a versatile carrier system. Enzymatic digestion of an internally quenched fluorescent peptide, covalently bound to the microspheres was successfully attempted. Those microspheres were used to transport a membrane impermeable dye into the intracellular space which was released once internalised, suggesting that the synthesised aminomethyl polystyrene microspheres are a practical delivery device for cellular delivery of normally impermeable compounds followed by their cellular release.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available