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Title: Use of non-exhaustive extraction techniques to assess bioavailability of PAHs in soil
Author: Patterson, Colin
ISNI:       0000 0001 3477 4440
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2006
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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants with both natural and anthropogenic sources. They have a tendency to persist for extended periods of time in a soil environment. In addition to this, they are toxic, mutagenic and carcinogenic, not only to humans but also to wildlife. A group of 16 PAHs have been highlighted by the United States Environmental Protection Agency (USEPA) and the European Union as being within the terminology of ‘Priority Pollutants’. Non-exhaustive extraction techniques (NEETs) offer a potential extraction system hat reflects the putatively bioavailable fraction of PAHs in soil. NEETs also offer the possibility of predicting an end-point for a remediation strategy. Previous techniques that have been used for determining the PAH load in contaminated soil have involved harsh exhaustive extraction techniques. It is now acknowledged that these methods are a poor reflection of the bioavailable fraction of PAHs in a soil environment. In this study biosensor analysis was used to complement the NEETs. Bioassays, using E. coli HMS174 and HPCD extracts, were used to determine if a link could be made between a chemical extraction and a biological assay. The results from this QSAR would suggest that the HPCD molecules are ‘masking’ the aromatic hydrocarbons from the biosensor and preventing full induction. In order to justify the use of NEETs, there is a requirement for them to be tested on environmentally relevant samples. In this study, NEETs were tested on different soils, including an industrially aged gas works soil, to determine if they could be used to indicate if and when a remediation end-point has been achieved.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available