Use this URL to cite or link to this record in EThOS:
Title: The clinical & immunological characteristics of kiwi fruit allergy in the UK
Author: Lucas, Jane Sarah Anne
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 2005
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
The results of this study confirmed that kiwi should be considered a significant food allergen, capable of causing severe reactions, particularly in young children. DBPCFC confirmed allergy to kiwi fruit in 53% of the subjects tested, who had a pervious history suggestive of kiwi allergy. Skin testing with fresh fruit has good sensitivity (93%), but poor specificity (45%) in this population. CAP slgE and a commercially available skin test solution were both much less sensitive (54%; 75%) but had better specificity (90%;67%. Patients with allergy to pollens were as likely to have severe kiwi allergy as those without. Inhibition immunoblots and ELISAs showed that cross reactivity with pollens is not common in this UK population. This was the first study to demonstrate that patients with oral allergy syndrome react to digestion labile epitopes, in contrast to patients with systemic symptoms whose IgE binds to digestion stable epitopes. Pepsin digestion of kiwi fruit in vitro was effected by minor changes in acidity, presumably affected the sensization capacity of the proteins. Western blotting to kiwi fruit protein extract revealed twelve proteins which were bound by IgE. A protein with a molecular weight of 38 kDa was the major allergen in this population, recognized by 59% of the population. No IgE bound to actinidin (Act c1), in kiwi protein extract, purified native or recombinant forms of actinidin during Western blotting. Pooled sera bound to kiwi protein extract but not purified actinidin on ELISA. Pre-incubating sera with actinidin did not inhibit IgE binding to kiwi protein extract on immunoblots or ELISA. This confirms that kiwi fruit contains multiple allergens, but actinidin, previous reported as the major kiwi fruit allergen, is not one of them in this large study population.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available