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Title: Investigation of supercritical fluid technology to produce dry particulate formulations of antibody fragments
Author: Sarup, Louise Santha
ISNI:       0000 0001 3553 3905
Awarding Body: University of London
Current Institution: University College London (University of London)
Date of Award: 2004
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Micro, submicrometre and ultrafine particles are increasingly in demand as they have a broad application in pharmaceutical delivery and needle-free powder injection. Conventional methods of powder preparation including freeze-drying and spray drying have considerable disadvantages for these applications. The use of supercritical fluids for materials' processing is one of the efficient and novel approaches used to achieve high purity micron-sized particles in a single step. The technique of Solution Enhanced Dispersion by Supercritical Fluids (SEDS) dramatically reduces or eliminates the problems associated with current, conventional particle-formation and size-reduction processes. SEDS involves the rapid dispersion, mixing and extraction of a protein solution, anti-solvent and supercritical carbon dioxide. SEDS has been used to produce dry particulate formulations of three antibody fragments (D1.3Fv, 4D5Fab, PEG-4D5Fab) and a whole antibody (CDP850). The aim of this research was to assess affects of the SEDS process on the functionality antibody fragments and antibody. The results demonstrated that dried formulations of antibody fragments and antibody can be produced using the SEDS process. The level of damage caused during SEDS was found to be dependent on choice solvent and protein. The main cause of activity loss was identified as the presence of both water and solvent during SEDS processing.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available