Use this URL to cite or link to this record in EThOS:
Title: Topographic reactions of cells in tissue repair
Author: MacDonald, Karen
ISNI:       0000 0001 3615 0084
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1997
Availability of Full Text:
Access from EThOS:
Access from Institution:
In this study, cells have been grown on micro- and nano-fabricated silica grooved structures in order to investigate further the mechanism of contact guidance, and with a view to producing a possible implant to guide tissue regrowth in wound healing. Previous work had shown that when cells are grown on microgrooved structures they align parallel with the grooves and the actin cytoskeleton of the cells aligns in a similar way. Results from this study have shown ordered staining of phosphorylated tyrosine along groove/ridge boundaries (on micro- and nanogrooved structures), suggesting a possible role for tyrosine phosphorylation in the mechanism that guides cell alignment on grooved substrates. It would appear that P388D1 macrophage-like cells show greater sensitivity to topographical cues than other cell types investigated, as these cells were shown to react to grooves of a depth as shallow as 44nm. Grooved structures were also fabricated in a biodegradable polyester, polydioxanone suture material (PDS), thought to be a suitable material for use as an implanted device. In vitro, cells reacted to these grooves in the same way as silica grooved structures. When the biodegradable structures were implanted intramuscularly in rats no adverse tissue reaction was observed, and the polydioxanone absorption profile remained normal. From histological and Scanning Electron Microscopy (SEM) studies, it was not possible to determine whether cells are oriented by the polydioxanone grooves in vivo. Messenger RNA fingerprinting experiments have also shown that cells grown on grooved substrates show differential gene expression compared with cells grown on flat substrates. Bands expressed in samples from cells grown on grooved substrates, but not in samples from cells grown on flat substrates, were excised from gels then cloned and sequenced.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Genetics