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Title: A study of the genetics and growth of a tripartite RNA virus using ts mutants
Author: Hampson, Jane
ISNI:       0000 0001 3529 0675
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 1987
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The growth characteristics of Maguari virus and Bunyamwera virus of the family Bunyaviridae were investigated using ts mutants and revertants. By genetic analysis involving recombination by segment reassortment between viruses, it was possible to assign Group I ts mutants to the S RNA, Group II to the M RNA and Group III to the L RNA segments. Representatives of each of these groups of mutants showed different phenotypes, which presumably reflect the effects of the ts lesions on the different virus functions encoded by each genome segment. It was found that Maguari virus dominates over Bunyamwera virus in mixed infections and this probably accounts for the predominance of Maguari genotype isolates from genetic crosses. Examination of a mixed reassortant clone indicated that multiple copies of at least the S RNA segment can exist in a virus preparation in a more intimate relationship than a simple mixture of two populations of viruses. Analysis of Group II revertants with altered G1 protein electrophoretic mobility provided information on the glycosylation of wild type G1 protein. It was found that the growth of these revertants in tissue culture was not defective despite the gross changes in a major structural protein. However, it was found that antiserum which neutralised the wild type virus failed to neutralise infectivity of one of the revertants.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QR Microbiology