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Title: The mechanism of uptake and intracellular fate of leupeptin in rat yolk sacs
Author: Clark, Susan A.
ISNI:       0000 0001 3556 586X
Awarding Body: Keele University
Current Institution: Keele University
Date of Award: 1986
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The aim of the work described in this thesis was to determine the mode of uptake of the tripeptide proteinase inhibitor leupeptin, in order to better interpret the effect of leupeptin on protein degradation within cells. Different methods of leupeptin detection were developed to monitor various aspects of the accumulation and action of leupeptin within yolk-sac tissue. The rate of accumulation, intracellular fate, and degree of inhibition of intracellular proteinases afforded by leupeptin, were all determined. The major route of entry of leupeptin into yolk sacs appeared to be fluid phase pinocytosis, though other modes, such as permeation, could not be entirely dismissed. Intracellular active leupeptin reached a steady state concentration that was capable of fully inhibiting lysosomal cathepsins B+L, though did not completely inhibit degradation of 125 I-BSA fd. Leupeptin was able to permeate into isolated intact lysosomes.
Supervisor: Williams, K. E. Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QH301 Biology