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Title: An investigation into the microbial bioconversion of cellulosic waste
Author: Mitchell, Anthony Frank
ISNI:       0000 0001 3411 2383
Awarding Body: Lancashire Polytechnic
Current Institution: University of Central Lancashire
Date of Award: 1986
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A low technology fermentation system for biodegradation of cellulose based waste material is described. Using a substrate of thermally bonded paper plastic laminate in a defined mineral salts medium the cellulolytic nicrofungus Trichoderma viride XXI 2% 458 was shown to effect the separation of the laminate into its components by enzymic degradation of the paper. This aerobically induced separation occurred within 14 days compared to upto 90 days by purely physical means. Growth of the fungus within the fermentation system was found to provide conditions that control the growth of contaminating microorganisms. Carbonolytic enzyme activity of Trichoderma viride was investigated by measurenent of substrate weight loss and by reduction in the viscosity of soluble substrate analogues when acted upon by filtrate front-the fermentation systais. An investigation of the effect of changes in carbon to nitrogen ratios during fermentation indicated that maximum carbonolytic enzyme activity occurred at a carbon to nitrogen ratio of 0.6:1 within this system. An examination of three other cellulolytic fungi in order to assess their potential as biodegradative organisms within the fermentation system showed that under microthermophilic conditions only Acrenonium stricturn approached the biodegradative activity of Trichoderma viride at mesophilic temperatures. The products of fermentation by Trichoderma viride were examined; protein levels in the residual cellulolytic substrate following biodegradation of paper plastic laminate were determined and found to reach levels of up to 9% following 35 days fermentation. The amino acid composition of protein produced by Trichoderma viride when grown on a range of substrates was determined and found to contain a wide range of essential and non essential amino acids. An investigation of mycotoxins both in the culture medium and residual paper component was undertaken. Comparisons with eleven common mycotoxin standards revealed no positive identifications under the conditions investigated.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: C790 - Molecular biology, biophysics & biochemistry not elsewhere classified