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Title: The effect of dietary n-3 and n-6 PUFA intake on atheromatous plaque lipid composition
Author: Garry, Jennifer Mary Christina
ISNI:       0000 0001 3491 575X
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 2001
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The aims of this study were to determine the effects of moderate supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFA) on the fatty acid profile and oxidisability of plasma low density lipoprotein (LDL) and of lipid fractions isolated from atherosclerotic plaques from patients undergoing carotid endarterectomy. Patients (n = 54/group) were given placebo, sunflower oil (SO) or fish oil (FO) in capsules (6 g of oil/day) for at least two weeks (mean eight weights) before undergoing surgery. The FO capsules provided 0.86 g eciosapentaenoic acid (EPA) and 0.5 g docosahexaenoic acid (DHA) per day. Blood samples were taken before and after supplementation. Copper-induced oxidation of isolated LDL (conjugated diene formation) and the fatty acid composition of lipid classes from LDL and from plaques were determined, as well as LDL and plasma triacylglycerol (TAG), cholesterol and lipid peroxide concentrations. FO supplementation increased the amount of EPA and DHA in LDL and plaque, phospholipid (PL), TAG and cholesteryl ester (CE) fractions, and in adipose tissue. Supplementation with SO resulted in increased levels of linolenic acid in LDL PL and CE fractions. Plasma TAG concentration decreased by 29% and the lag time of copper induced-LDL oxidation was significantly decreased (-26%) after FO supplementation. Peroxide levels in LDL were not affected by any of the supplements. The incorporation of n-3 PUFA into plaques led to decreased levels of macrophages. The results of this study show that a moderate supplementation of fish oil in patients with carotid atherosclerosis results in incorporation of the long chain n-3 PUFA (EPA and DHA) into LDL fractions, adipose tissue and atherosclerotic plaques.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Cardiovascular disease; Lipids; Fatty acid