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Title: The effect of the viscosity of the medium on the reactions of cells to topography
Author: Khorshid, Faten
ISNI:       0000 0001 3598 7210
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 2001
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The viscosity of the medium in which cells grow may alter their cellular reaction to topography and this in turn may affect cell growth, morphology and movement. In this study, cells have been grown in medium with high viscosity on plane and grooved topography in order to investigate the effect of the viscosity of the medium on the reaction of cells to topography. The viscosity was changed by adding viscous macromolecules, such as dextran, ficoll, polyvinylpyrrolidone, Methylcellulose and Carboxymethylcellulose to the normal medium (ECT or Ham's F10). Due to other solution properties that might be altered by the change of medium viscosity, such as osmolarity or chemical interactions, cells were examined in the presence of a variety of concentrations of macromolecules, which differed in their size, capacity to elevate fluid viscosity, and in their chemical nature. The medium viscosity was determined by measuring its flow time, relative to water, using an Ostwald viscometer. All measurements were performed at 37°C. A novel feature of these studies is combining measurement of the medium viscosity with the osmolarity of all media used in the experiments. The osmotic effect could be discounted. Diffusion was altered when the viscosity of the medium was raised, which in turn affects the reactions of cells to topography. Results from this study have shown that the cell morphology is directly affected when cells are grown in medium of high viscosity. In other words, increasing the medium viscosity decreases the cell length. Increasing the viscosity also reduced cell motility. It also seems that medium viscosity affects the cytoskeleton by limiting its assembly and operation. Cells were also examined in the medium of high viscosity by ordinary IRM and Live IRM with associated of the refractive index measurements. Results from IRM study show that the space available for diffusion under the cells was decreased when the viscosity of the medium was raised. Recovery experiments have shown that were cells still alive in the medium of high viscosity and after they recovered in ordinary medium they spread and aligned again. The results support the hypothesis that diffusion effects around the cells may be important in the reaction of cells to topography. The results of the present study suggest that elevated medium viscosity is pertinent to biochemical and cellular functions of cells (e.g. plasma and blood cells).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Medicine