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Title: In vitro biosynthesis of 1,4-β-galactan attached to a pectin-xyloglucan complex in peas
Author: Abdel-Massih, Roula M.
ISNI:       0000 0001 3389 4213
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 2001
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Cell-wall matrix polysaccharides are synthesised in the Golgi apparatus and then transported to the cell wall. We have investigated the properties of nascent pectin formed by the biosynthetic system in vitro. Particulate enzyme preparations were prepared from etiolated pea epicotyls (Baydoun et al, 2001) and used to assay for 1,4-beta-galactan synthase using UDP-[U-14C]galactose. Optimum conditions for 1,4-beta-galactan synthesis were determined. The enzyme products were characterised by selective enzymatic degradation and anion-exchange chromatography. Evidence was obtained for the formation of 1,4-beta-galactan chains attached to a pectic backbone containing both polygalacturonic acid and rhamnogalacturonan I. The results also indicated that part or all of this nascent pectin was present as a complex with xyloglucan. This complex may be similar to pectin-xyloglucan complexes found in the cell wall in rose suspension cells (Thompson and Fry, 2000) and cauliflower stem (Femenia et al., 1999).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Plant cell wall components