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Title: Regulation of metallothionein isoform expression following induction by metals
Author: Vasconcelos, Maria Helena da Silva de
ISNI:       0000 0001 3543 0148
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1996
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The work described in this thesis reports the specificity of expression, and regulation of expression, of each rat MT isoform. Subcutaneous (sc) injection of 8.9 μmol/kg of cadmium (Cd) rapidly induced MT-1 (10-fold) and MT-2 (3-fold) total mRNAs in rat liver. However, at the peaks of the mRNA induction, not all the MT-2 mRNA was recruited into polyribosomes for translation, suggesting that there is post-transcriptional regulation of MT-2 expression. No evidence was found for such regulation of the MT-1 isoform. In rat kidneys, both isoform mRNAs were rapidly induced (10-fold) but there was no evidence for a similar induction of MT-protein, suggesting that there is post-transcriptional regulation of both MT isosforms in the kidneys. In both organs, metal analysis of digested tissues and gel chromatography/ICP-MS profiles indicated that there was an increase in Cd and Cd-Mt respectively. Subcutaneous (sc) injection of 8.7 μmol/kg of copper (Cu) rapidly induced both MT isoform mRNAs in liver. The extent of induction of total mRNA was higher for MT-2(30-fold) than for MT-1 (10-fold). As in the case of the Cd study, there was evidence for post-transcriptional regulation of MT-2 synthesis, but not of MT-1, according to analysis of polyribosomal mRNA. In the kidneys, there was no evidence for de novo synthesis of either MT mRNAs. However, there was a small induction of MT-protein. This may be explained by the transport of MT from other organs into the kidney under these treatment conditions. In both organs, an increase in Cu concentration and Cu-MT content was found.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Biochemistry