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Title: The role of bacterial surface polymers in the adhesion of freshwater bacteria to solid surfaces
Author: Pringle, James Howard
ISNI:       0000 0001 3501 817X
Awarding Body: University of Warwick
Current Institution: University of Warwick
Date of Award: 1984
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In the study of the mechanism of non-specific permanent adhesion of bacteria at the solid-liquid interface a number of alternative approaches have been used. These include; the use of thermodynamic models, to explain observations of biocontact phenomenal the use of disruptive chemicals, enzymes and electronnicroscopy to investigate the nature of the adhesive bond and finally the biochemical analysis of cell surface components possibly involved in the process. In this study all three approaches were combined in an investigation of the role of cell surface components on the attachment of freshwater organisms to solid surfaces. A thermodynamic relationship was found using attachment assays on a number of freshwater isolates emphasising the influence of water on the adhesive process. Further investigations of phenotypic changes in membrane surface composition of a number of Pseudomonas isolates using continuous culture demonstrated the involvement of polysaccharide in the inhibition of adhesion. A number of adhesion mutants were analysed for genotypic changes in outer membrane proteins, lipopolysaccharides and exopolysaccharides which also confirmed the inhibitory role of polysaccharides. The results demonstrate the role of cell surface characteristics in the adaptability of the organism to micro-environments such as a solid/liquid or air/liquid interface or the aqueous phase.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QR Microbiology