Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.347613
Title: Plant regeneration from protoplasts of Solanum tuberosum and Solanum brevidens
Author: Nelson, Robin Stephen
ISNI:       0000 0001 3441 3530
Awarding Body: University of Bath
Current Institution: University of Bath
Date of Award: 1983
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
Abstract:
The use of in vitro techniques as a supplement to conventional methods for potato breeding is discussed. The literature on protoplast, isolation, culture and somatic hybridisation is reviewed. The materials and methods used are described. Meristem-tip cultures of three S. tuberosum lines were used to initiate shoot cultures. Plants grown under different environmental regimes were assessed as protoplast sources. Isolations from shoot cultures were more reproducible but gave lower protoplast yields than leaves from growth room grown plants. Protoplasts from a tetraploid cultivar (Fortyfold) from both shoot cultures and leaves of whole plants were successfully cultured and regenerated to plants. Isolated protoplasts from two monohaploid lines failed to respond to the culture conditions used. Shoot cultures of S. brevidens were suitable for the isolation of high yields of viable protoplasts, from which fertile plants were regenerated. Isolated protoplasts of S. brevidens were used in attempts to improve the plating characteristies of cultured Solanum protoplasts. A protocol enabling the culture of protoplasts at low population densities and subsequently, more efficient regeneration of plants from protoplast-derived calluses was developed. The final protocol was applied to the culture of protoplasts from a range of S. tuberosum genotypes. Plants regenerated from protoplasts of S. brevidens and S. tuberosum showed phenotypic variation. Cytological analysis revealed some of this variation correlated with changes in ploidy, aneuploidy and chromosome structural changes. Variation was also present among plants with the normal chromosome number. Unstable changes in the phenotype of regenerated plants were also observed. The final protocol described in this thesis is discussed in perspective with other methods for protoplast culture. The uses of potato protoplasts are discussed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.347613  DOI: Not available
Keywords: Human anatomy & human histology
Share: