Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.346255
Title: Gene transfer in Bordetella pertussis
Author: Al-Sallami, Saleh Fadhel Mohsin
ISNI:       0000 0001 3408 4992
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 1981
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Abstract:
In order to have available a procedure for gene transfer in Bordetella pertussis, the transformation system described originally by Branefors (1964) was re-examined and further developed. Under her original conditions, few streptomycin-resistant transformants were obtained in the present investigation when DNA prepared from the 44122/7R streptomycin-resistant strain was incubated with competent 44-122/7 streptomycin-sensitive cells. In attempts to improve the transformation frequency, conditions for DNA isolation, preparation of competent cells and exposure of recipient cells to DNA were examined in detail.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.346255  DOI: Not available
Keywords: Genetics
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