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Title: A study of selected isoenzymes in sheep
Author: Beatty, Elspeth M.
ISNI:       0000 0001 3452 7327
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1983
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The work described in this thesis involved a study of isoenzymes (multiple forms of enzymes arising from genetically determined differences in primary structure) in ovine tissues and serum. The study is divided into three major parts. The first part describes the technical problems encountered and the methods used to solve these problems in relation to separating the isoenzymes of lactate dehydrogenase, glucosephosphate isomerase, aldolase, aspartate aminotransferase and creatine kinase. Both isoelectric focusing and electrophoresis were investigated but only the latter proved suitable for the quantitative estimations described in parts two and three. In the second part, normal isoenzyme levels were established in ovine tissues and an extensive investigation, involving 151 sheep, was carried out to determine the effect of physiological parameters on serum isoenzyme levels. Time after parturition in ewes and age in lambs affected serum lactate dehydrogenase isoenzyme levels but sex and growth rate produced no effects of any clinical significance. In a separate experiment, the frequency and mode of inheritance of two genetic variants of glucosephosphate isomerase was investigated. In the third part, the serum and tissue levels of lactate dehydrogenase and creatine kinase were studied in respiratory and gastrointestinal tract disease. In chronic proliferative exudative pneumonia and in acute pasteurella pneumonia, no changes in serum isoenzyme levels were observed which were likely to be of diagnostic value, despite a marked increase in the total lactate dehydrogenase level and changes in the distribution of its isoenzymes in pneumonic lungs. In chronic gastrointestinal parasitism, changes in serum lactate dehydrogenase and creatine kinase isoenzyme levels attributable to damage to the intestinal tract caused by Trichostrongylus vitrinus were detectable and may be of value in following the course of experimental infections in the live sheep. In the acute and chronic stages of parasitic gastritis caused by Ostertagia circumcincta or Ha emonchus contortus, no diagnostically useful serum isoenzyme changes were observed. In acute ID. circumcincta infection, isoenzyme levels in serum, and lymph from the gastric lymph duct were measured. Lymph isoenzymes were no more sensitive than serum isoenzymes in detecting damage to the abomasal mucosa, suggesting that the isoenzymes may have been released from the mucosa into the lumen of the abomasum rather than into the circulation via the lymphatic drainage Changes in the lactate dehydrogenase isoenzyme distribution of the abomasal mucosa were observed in acute parasitic gastritis but these were not reflected in the serum isoenzyme levels.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Human anatomy & human histology