Use this URL to cite or link to this record in EThOS: | https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338340 |
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Title: | Defining HLA-A locus alleles from DNA using ARMS-PCR | ||||||
Author: | Krausa, Peter |
ISNI:
0000 0001 3602 423X
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Awarding Body: | Open University | ||||||
Current Institution: | Open University | ||||||
Date of Award: | 1997 | ||||||
Availability of Full Text: |
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Abstract: | |||||||
The HLA system is a highly polymorphic group of genes which play a central role in the immune response. The definition of the HLA system makes an important contribution to a number of fields. In the clinical setting it is a requirement for transplantation. For anthropology, the HLA system is a useful marker for assessing population groups. Functionally, it is an important component of the T cell mediated immune response. The definition offered by the established method of serology to HLA class I tissue-typing is restrictive since serology fails to discriminate between alleles which are functionally distinct. This research represents one of the first DNA based approaches and the first by PCR-SSP, to define HLA class I specificities, with particularly reference to the HLA-A locus. This thesis charts both the development and application of a robust, simple to perform, easy to interpret yet highly powerful PCR based system in which allelic definition is demonstrated. In addition to resolution, a DNA approach offers many practical advantages over the restrictive demands of a serological typing. Further more, this thesis examines the functional aspect of polymorphism, in defining the peptide binding preferences of a group of closely related HLA specificities. By so doing, this thesis shows the relevance of a highly definitive approach to HLA typing.
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Supervisor: | Not available | Sponsor: | Not available | ||||
Qualification Name: | Thesis (Ph.D.) | Qualification Level: | Doctoral | ||||
EThOS ID: | uk.bl.ethos.338340 | DOI: | |||||
Keywords: | Genetics | ||||||
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