Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286982
Title: Superheated water as a mobile phase
Author: Burgess, Robert John
ISNI:       0000 0001 3509 7154
Awarding Body: Loughborough University
Current Institution: Loughborough University
Date of Award: 1999
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Abstract:
This project has focused on the applicability of using superheated water as an alternative eluent for use in reversed phase high performance liquid chromatography (RP-HPLC). A system based on instrumentation from gas chromatography (GC), HPLC, and supercritical fluid chromatography (SFC) was utilised with some success. Of particular importance was the stability of the column stationary phases commonly employed in RP-HPLC. The most popular phases based on alkyl bonded silica proved unsuitable due to excessive dissolution of the base silica at high temperatures. Nevertheless, two other phases—one a poly(styrene-divinylbenzene) material and the other a porous graphitic carbon—proved stable to temperatures as high as 240°C. At such temperatures compound stability was good, except for a notable case with nitrobenzene. The mechanism of elution remained reversed phase mode throughout, with polar solutes being eluted before non-polar ones.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.286982  DOI: Not available
Keywords: Gas chromatography; SFC; HPLC
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